Fig. 6

Effect of Electrical stimulation on ASCs and their secretome on hNPCs. (A-B) Diagram of Experimental Design and Groups - Effect of Secretome from Electrically Stimulated ASCs on hNPCs. ASCs were cultured until reaching 80% of confluence and them electrically stimulated for 30 min. Then ASCs were allowed to grow for 24 h in serum-free medium for collecting the conditioned medium. The secretome of ASCs were then incubated with hNPCs for 24 h, and cells were then fixed for immunoimaging analysis. (Image created with Biorender). (C) Representative fluorescence microscopy images of ASCs when electrically stimulated and control group. Scale bar: 100 μm. (D) Represents the population of DAPI + cells. (E) Represents the circularity of the cells based on the morphology of phalloidin staining. (F) Correlation between shape and circularity of cells. (G) Representative fluorescence microscopy images of hNPCs when incubated with secretome from electrically stimulated ASCs. Scale bar: 50 μm. (H) Represents the population of DAPI + cells. (I) Represents the average neurite length per neuron. (J) Represents the population of MAP-2 + cells relative to the population of DAPI + cells. (K) Represents the population of DCX + cells relative to the population of DAPI + cells. ’S’ denotes the group with non-stimulated secretome. ’ES’ denotes the group that was electrically stimulated. *p value < 0,05; **p value < 0,01; ****p value < 0,0001. Data are show as mean ± SEM