Fig. 4

Electrical stimulation induces cellular and neuronal morphology changes. (A) Diagram of experimental design – Effect of Electrical Stimulation on mixed cell culture. Cells were cultured for 12 days and were then stimulated for 30 min on two consecutive days. After 14 days, the cells were either fixed for immunoimaging analysis or their metabolic activity was assessed using the MTS assay. (Image created with Biorender). (B) MTS to evaluate the metabolic status of the culture. (C) Total number of cells. (D) Neurite length was measured manually for each neuron. (E) The total area occupied by GFAP positive area normalized for the total cells. (F) The total area occupied by Nestin positive area normalized for the total cells. (G) Quantification of the percentage of Oligo-2 positive cells relative to total cells. (H) Representative immunofluorescence images of primary cultures of spinal cord cells at day 7 for BIII tubulin. n = 8. Scale bar: 50 μm. *p value < 0,05; **p value < 0,01; ***p value < 0.001; ****p value < 0,0001; Data are show as mean ± SEM