Fig. 3

Different electrical stimulation settings impact SH-SY5Y neuronal cells. (A) Diagram of experimental design – Effect of Electrical Stimulation on SH-S5Y5. Cells were cultured for 12 days to allow differentiation into neurons and were then stimulated for 10 min on two consecutive days. After 14 days, the cells were either fixed for immunoimaging analysis or their metabolic activity was assessed using the MTS assay. (Image created with Biorender). (B) In vitro electrical stimulator settings. (C) Representative fluorescent microscopy images of SH-SY5Y cells, electrical stimulated and non-stimulated, neurites stained with BIII tubulin (green) and nuclei with dapi (blue). (D) Metabolic assessment of the cell cultures by MTS assay. (E) Cell number quantification at day seven. (F) Quantification of the individual cell neurite length. Two independent experiments, n = 8. Data are show as mean ± SEM. *p value < 0,05; **p value < 0,01; ****p value < 0,0001. Scale bar: 40 μm