Fig. 1

Eagle imaging device. a Spectral diagram of FL optical components and PpIX excitation and emission spectra. The primary PpIX excitation peak is targeted with 405 nm LEDs. The imaging filter transmits green (500 to 550 nm) and red (600 to 660 nm) wavelengths to the camera. b Front (user-facing) and c rear (target-facing) views of the Eagle FL prototype device. The WL device is physically identical aside from the removal of the emission filter at the imaging tip. The user-facing side features a built-in touchscreen display, which is used to capture and view images and videos. The optics are mounted on the target-facing side at the distal tip of an aluminum shaft, which is sized to reach into and image all surfaces of surgical cavities. d Imaging tip of the Eagle FL device showing relative positions of the LEDs and camera. The diameter of the imaging tip is 24 mm. e Eagle imaging stand used to mount the Eagle device for consistent, reproducible ex vivo imaging. The Eagle prototype, mounted in the 3D printed custom mount, sits with the optics pointed directly downward at the imaging sample. A disposable, sterile black imaging sheet (not pictured) is taped down to the optical board when imaging biological tissues to avoid contamination of the sample and optical board. The imaging distance can be adjusted by sliding the mounting bracket along the optical post and measuring the distance between the Eagle device’s tip and the sample. The post is graduated and keyed to prevent rotation of the mounting bracket